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Human CRISPR Inhibition Pooled Library (Katsano)
(Pooled Library #260975)

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  • Purpose

    Katsano is a human genome-wide Cas9 CRISPR interference library to be used for loss-of-function genetic screening. Each protein-coding gene is targeted by three constructs, each expressing a single guide RNA. The library was designed to be cell-line agnostic. Katsano was designed to prioritize highly effective guides as predicted by Rule Set 3 Interference, an up-to-date model for CRISPRi-specific guide RNA activity prediction, and avoid guides with seed sequences observed to promote off-target activity. Furthermore, Katsano targets recently annotated transcription start sites (TSSs) reflective of GENCODE v48 (Ensembl v114), including the entire MANE Select set and a handful of alternative promoters.

  • Vector Backbone

    pMV_AA050 (Plasmid #216107) - does not express dCas9

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 260975 gRNA pooled library† 1 $642 Add to Cart
Available to Academic and Nonprofits Only

  • † A dCas9 plasmid is NOT included with this item and will have to be ordered separately. Can be used in conjunction with pRDB_332 (Addgene #260891) or any other plasmid(s) or cell lines expressing a dCas9-KRAB fusion construct.

Library Details

  • Species
    Human
  • Genes targeted
    21,609 transcription start sites
  • gRNAs
    63,404
  • Controls
    900 intergenic-targeting, 100 non-targeting
  • Lentiviral generation
    3rd

Library Shipping

Each library is delivered in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.

  • Volume
    ∼25 µL
  • Concentration
    50 ng/µL

Resource Information

Depositor Comments

The following design process was repeated for each target transcription start site (TSS): 1. Identify candidate guides: NGG-adjacent sites within 50 base pairs upstream and 300 base pairs downstream of the TSS. 2. Remove guides with 10 or more off-target sites. 3. Rank guides by potential for on-target activity (RS3i Score). 4. Pick the top guide that satisfies the following criteria: Seed Score less than four; RS3i Score greater than 1.2; no off-target sites near protein-coding TSSs; no off-target sites; Seed Score less than three; and low SNP frequency at target site. This last step is repeated for three guides per TSS, relaxing the criteria (prioritized in the order listed) as needed.
Figure 1: Overview of the Katsano library design.
How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Human CRISPR Inhibition Pooled Library (Katsano) was a gift from David Root and John Doench (Addgene #260975 ; http://n2t.net/addgene:260975 ; RRID:Addgene_260975)

  • For your References section:

    Optimized parameters for CRISPR-Cas9 interference library design. Srikanth S, Zheng F, Drepanos LM, Shah ST, Kaplan EG, Gibson D, Lynch GO, Uebele AT, Reint G, Merzouk S, Doench JG. Cell Genom. 2026 Aug 28:101338. doi: 10.1016/j.xgen.2026.101338. PubMed 42664970
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