pBCN27-Pmyo-2::GFP::unc-54_3'UTR
(Plasmid #26347)

• Depositing Lab: Ben Lehner
• Publication: Semple et al Nat Methods. 2010 Aug 22. ():.

Backbone

• Vector backbone: pBCN27-R4R3
• Backbone size w/o insert (bp): 10813
• Vector type: Worm Expression
• Selectable markers: Puromycin ; unc-119

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Pmyo-2::GFP::unc-54_3'UTR
• Species: C. elegans (nematode)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AttR4 (destroyed during cloning)
• 3′ cloning site: AttR3 (destroyed during cloning)
• 5′ sequencing primer: n/a

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Puromycin resistance gene from pBabePuro (Addgene). rpl-28 promoter and let-858 3'UTR sequences from pPD129.57 vector (Addgene).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The full sequence provided is theoretical sequence based on a three way gateway reaction. The order of the elements has been confirmed by PCR, but not fully sequence verified. Expression pattern in worms is as expected.

Use this plasmid as a positive control for testing puromycin selection after single copy insertion by MosSCI at the ttTi5605 locus.