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(Plasmid #26686)


Item Catalog # Description Quantity Price (USD)
Plasmid 26686 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *

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  • Vector backbone
  • Backbone size (bp) 6900

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
    In HB101 cells. Use 50ug/ml Amp
  • Copy number


  • Gene/Insert name

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer M13pUC-fwd
  • 3′ sequencing primer M13rev
  • (Common Sequencing Primers)

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

To construct pKSV7, pUCI8 [28] was partially
digested with PvulI and ligated to pBD95ts [22, 33]
linearized with Pvull (fig 1). A ligation product having
pBD95ts inserted into the PvulI site external to the
lacZ sequences was designated pKSV4; this preserves
the ability to screen for alpha-complementation when
cloning into the polylinker site. A HindlII-EcoRl
fragment containing the ble gene encoding bleomycin/
phleomycin resistance was inserted into the
HindlH-EcoRl backbone of pKSV4, replacing the
polylinker and resulting in a unique Pstl site. This Psd
site was removed by digestion with Pstl followed by
treatment with T4 DNA polymerase. The polylinker
was restored to produce pKSV7.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKSV7 was a gift from Philip Youngman (Addgene plasmid # 26686 ; ; RRID:Addgene_26686)
  • For your References section:

    Use of a new integrational vector to investigate compartment-specific expression of the Bacillus subtilis spoIIM gene. Smith K, Youngman P. Biochimie. . 74(7-8):705-11. 10.1016/0300-9084(92)90143-3 PubMed 1391050