|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||26686||Standard format: Plasmid sent in bacteria as agar stab||1||$75 *|
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- Backbone size (bp) 6900
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth instructionsIn HB101 cells. Use 50ug/ml Amp
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer M13pUC-fwd
- 3′ sequencing primer M13rev (Common Sequencing Primers)
Terms and Licenses
- Zeocin® is an InvivoGen trademark.
To construct pKSV7, pUCI8  was partially
digested with PvulI and ligated to pBD95ts [22, 33]
linearized with Pvull (fig 1). A ligation product having
pBD95ts inserted into the PvulI site external to the
lacZ sequences was designated pKSV4; this preserves
the ability to screen for alpha-complementation when
cloning into the polylinker site. A HindlII-EcoRl
fragment containing the ble gene encoding bleomycin/
phleomycin resistance was inserted into the
HindlH-EcoRl backbone of pKSV4, replacing the
polylinker and resulting in a unique Pstl site. This Psd
site was removed by digestion with Pstl followed by
treatment with T4 DNA polymerase. The polylinker
was restored to produce pKSV7.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pKSV7 was a gift from Philip Youngman (Addgene plasmid # 26686 ; http://n2t.net/addgene:26686 ; RRID:Addgene_26686)
For your References section:Use of a new integrational vector to investigate compartment-specific expression of the Bacillus subtilis spoIIM gene. Smith K, Youngman P. Biochimie. . 74(7-8):705-11. 10.1016/0300-9084(92)90143-3 PubMed 1391050