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pEGFPc2 mAbp1
(Plasmid #26719)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 26719 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-C2
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Actin-binding Protein-1
  • Alt name
    mAbp1
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Dbnl (a.k.a. RP23-385C16.5, Abp1, SH3P7, mAbp1)
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer EGFP-C
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Mouse mAbp1 was kindly provided by David Drubin (University of California-Berkeley)
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFPc2 mAbp1 was a gift from Anna Huttenlocher (Addgene plasmid # 26719 ; http://n2t.net/addgene:26719 ; RRID:Addgene_26719)
  • For your References section:

    Actin-binding protein-1 interacts with WASp-interacting protein to regulate growth factor-induced dorsal ruffle formation. Cortesio CL, Perrin BJ, Bennin DA, Huttenlocher A. Mol Biol Cell. 2010 Jan 1. 21(1):186-97. 10.1091/mbc.e09-02-0106 PubMed 19910490