|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||26774||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerStan Dunn
- Backbone size w/o insert (bp) 5696
Vector typeBacterial Expression
Growth in Bacteria
SpeciesH. sapiens (human)
Insert Size (bp)586
Entrez GeneS100B (a.k.a. NEF, S100, S100-B, S100beta)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer M13/pUC Reverse
- 3′ sequencing primer pBAD-rev (Common Sequencing Primers)
There are a few mismatches between author sequence and Addgene quality control sequence. These nucleotide differences do not alter the amino acid sequence or the function of the plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSS2 (hS100B) was a gift from Gary Shaw (Addgene plasmid # 26774 ; http://n2t.net/addgene:26774 ; RRID:Addgene_26774)
For your References section:Structural influence of cation binding to recombinant human brain S100b: evidence for calcium-induced exposure of a hydrophobic surface. Smith SP, Barber KR, Dunn SD, Shaw GS. Biochemistry. 1996 Jul 9. 35(27):8805-14. 10.1021/bi952698c PubMed 8688416