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pSS2 (hS100B)
(Plasmid #26774)


Item Catalog # Description Quantity Price (USD)
Plasmid 26774 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Stan Dunn
  • Backbone size w/o insert (bp) 5696
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Entrez Gene
    S100B (a.k.a. NEF, S100, S100-B, S100beta)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer M13/pUC Reverse
  • 3′ sequencing primer pBAD-rev
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

There are a few mismatches between author sequence and Addgene quality control sequence. These nucleotide differences do not alter the amino acid sequence or the function of the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSS2 (hS100B) was a gift from Gary Shaw (Addgene plasmid # 26774 ; ; RRID:Addgene_26774)
  • For your References section:

    Structural influence of cation binding to recombinant human brain S100b: evidence for calcium-induced exposure of a hydrophobic surface. Smith SP, Barber KR, Dunn SD, Shaw GS. Biochemistry. 1996 Jul 9. 35(27):8805-14. 10.1021/bi952698c PubMed 8688416