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lag-2 promoter:GFP (pJK590)
(Plasmid #26814)


Item Catalog # Description Quantity Price (USD)
Plasmid 26814 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Andrew Fire (Addgene Plasmid #1497)
  • Backbone size w/o insert (bp) 4492
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    lag-2 promoter
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
  • Tag / Fusion Protein
    • GFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer M13_Rev
  • 3′ sequencing primer GFP-R
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Quick confirmation: BamHI should result in two fragments: ~3kb and 4.5kb.

This is a lag-2 promoter::GFP fusion. Strong GFP in live worms. 3kb BamHI fragment of pJK520 is the complete 3kb 5' region of lag-2.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    lag-2 promoter:GFP (pJK590) was a gift from Judith Kimble (Addgene plasmid # 26814 ; ; RRID:Addgene_26814)
  • For your References section:

    APX-1 can substitute for its homolog LAG-2 to direct cell interactions throughout Caenorhabditis elegans development. Gao D, Kimble J. Proc Natl Acad Sci U S A. 1995 Oct 10. 92(21):9839-42. 10.1073/pnas.92.21.9839 PubMed 7568229