|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||26844||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 6036
Modifications to backboneTEF1 promoter and CYC1 terminator sequence were inserted into the unique SspI site of pUC18. Note that this cloning strategy split part of the ampicillin promoter, but the plasmid still confers ampicillin resistance in bacteria.
Vector typeYeast Expression, Cre/Lox
Growth in Bacteria
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ cloning site SpeI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer N/A (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pXP420 was a gift from Nancy DaSilva & Suzanne Sandmeyer (Addgene plasmid # 26844 ; http://n2t.net/addgene:26844 ; RRID:Addgene_26844)
For your References section:A vector set for systematic metabolic engineering in Saccharomyces cerevisiae. Fang F, Salmon K, Shen MW, Aeling KA, Ito E, Irwin B, Tran UP, Hatfield GW, Da Silva NA, Sandmeyer S. Yeast. 2010 Sep 10. ():. 10.1002/yea.1824 PubMed 20936606