pCI HA NEDD4
(Plasmid #27002)

• Depositing Lab: Joan Massague
• Publication: Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Backbone

• Vector backbone: pCI
• Backbone size w/o insert (bp): 4000
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NEDD4
• Species: H. sapiens (human)
• Insert Size (bp): 2700
• Entrez Gene: NEDD4 (a.k.a. NEDD4-1, RPF1)
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: EBV-rev

Resource Information

• Articles Citing this Plasmid:
  • 19 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry