pLIC
(Plasmid #27989)

• Purpose: (Empty Backbone)
• Depositing Lab: Zhihong Zhang
• Publication: Yang et al Biotechniques. 2010 Nov . 49(5):817-21.

Backbone

• Vector backbone: pLIC
• Backbone manufacturer: Jie Yang Lab
• Backbone size (bp): 3300
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 reverse

Resource Information

• Supplemental Documents:
  • pLIC cloning sequence

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid is used for a novel and straightforward ligation-independent cloning (LIC) method based on nicking DNA endonuclease (NiDE).

To construct a NiDE-compatible pLIC vector, LacZ cDNA was amplified from a modified pMD18 construct by PCR using primers Laz-BgiII-F and Laz-EcoR I-R. The β-galactosidase gene lacZ, including restriction endonuclease sites such as BamHI, SalI, PstI, and SphI, was amplified and then digested by BglII and EcoRI. It was then cloned into pRSETb plasmid between BamHI and EcoRI sites to generate the pLIC vector.

See Author's Map and published article for more information.