Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

FLX1.8MitoKaede
(Plasmid #28133)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 28133 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    FLX1.8CMV
  • Backbone size w/o insert (bp) 5652
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Stbl3
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    MitoKaede
  • Species
    Trachyphyllia geoffroyi
  • Insert Size (bp)
    782
  • GenBank ID
    AB085641
  • Tag / Fusion Protein
    • COX VIII signal sequence (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site MluI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    MBL International Corporation
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene provides this plasmid in Stbl3, but the depositing scientist recommends using MDS42recA
from www.scarabgenomics.com for further amplification.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FLX1.8MitoKaede was a gift from Geoffrey Owens (Addgene plasmid # 28133 ; http://n2t.net/addgene:28133 ; RRID:Addgene_28133)