Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||28192||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3900
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin and Kanamycin
Copy numberHigh Copy
Gene/Insert nameMCV replication region
Alt nameHuman Merkel Cell polyomavirus
SpeciesHuman Merkel Cell polyomavirus
Insert Size (bp)583
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site EcoRV (not destroyed)
- 5′ sequencing primer M13pUC-fwd
- 3′ sequencing primer M13rev (Common Sequencing Primers)
Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCR.MCV.Ori339 (589) was a gift from Patrick Moore (Addgene plasmid # 28192 ; http://n2t.net/addgene:28192 ; RRID:Addgene_28192)
For your References section:T antigen mutations are a human tumor-specific signature for Merkel cell polyomavirus. Shuda M, Feng H, Kwun HJ, Rosen ST, Gjoerup O, Moore PS, Chang Y. Proc Natl Acad Sci U S A. 2008 Oct 21. 105(42):16272-7. 10.1073/pnas.0806526105 PubMed 18812503
Map uploaded by the depositor.