pcDNA3.T7 NFAT3
(Plasmid #28223)

• Depositing Lab: Sebastien Jauliac
• Publication: Fougere et al Oncogene. 2010 Apr 15. 29(15):2292-301.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5469
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: human NFAT3
• Alt name: NFATC4
• Species: H. sapiens (human)
• Insert Size (bp): 2715
• Mutation: G160A
• GenBank ID: NM_004554
• Entrez Gene: NFATC4 (a.k.a. NF-AT3, NF-ATC4, NFAT3)
• Promoter: CMV
• Tag / Fusion Protein:
  • T7 (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Tim Hoey

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that Addgene's sequencing results identified a G160A mutation in the NFAT3 sequence when compared to GenBank reference NP_004545.2