|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||29327||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerPfeiffer et al
- Backbone size w/o insert (bp) 7831
Vector typeInsect Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesD. melanogaster (fly)
- Cloning method Restriction Enzyme
- 5′ cloning site Bgl II (not destroyed)
- 3′ cloning site Xba I (not destroyed)
- 5′ sequencing primer hsp70 F: GAGCGCCGGAGTATAAATAGAG
- 3′ sequencing primer SV40 R: CCATTCATCAGTTCCATAGG (Common Sequencing Primers)
Terms and Licenses
This plasmid contains mTFP1.0, and not EBFP2, as suggested by the linear plasmid map image file.
Please note that Addgene's sequencing results identified a few discrepancies when compared to GenBank ID JF267350. The depositing laboratory states that these differences are not a concern for the function of the plasmid. The full plasmid sequence has been assembled and not entirely confirmed by sequencing. Additional discrepancies may exist.
This plasmid was constructed using the following restriction enyzmes:
Bgl II / Not I - loxP sites
Not I / Xho I - EGFP-HSV
Xho I / Kpn I - mTFP1.0-HA
Kpn I / Xba I - mKO2-V5
NotI is not a unique cutter--it is present twice in the plasmid. The XbaI site has been confirmed by Addgene's sequencing results, but it not present in the full plasmid sequence.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:dBrainbow was a gift from Julie Simpson (Addgene plasmid # 29327 ; http://n2t.net/addgene:29327 ; RRID:Addgene_29327)
For your References section:Drosophila Brainbow: a recombinase-based fluorescence labeling technique to subdivide neural expression patterns. Hampel S, Chung P, McKellar CE, Hall D, Looger LL, Simpson JH. Nat Methods. 2011 Feb 6. ():. 10.1038/nmeth.1566 PubMed 21297621