pCS2+ Bmi1-cherry-HA
(Plasmid
#30516)
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Depositing Lab
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Depositing OrganizationWellcome Trust/Cancer Research UK Institute (Inactive)
Located in United Kingdom of Great Britain and Northern Ireland -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 30516 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 30516-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 30516-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCS2+ att-cherry-HA (C-term cherry-HA)
- Backbone size w/o insert (bp) 4918
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Vector typeMammalian Expression ; Xenopus/Zebrafish expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10/P3
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBmi1
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SpeciesM. musculus (mouse)
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MutationNO STOP
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GenBank IDNM_007552.4
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Entrez GeneBmi1 (a.k.a. Bmi-1, Pcgf4)
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Tags
/ Fusion Proteins
- cherry (C terminal on backbone)
- triple HA (C terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer SP6
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 30516-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 30516-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCS2+ Bmi1-cherry-HA was a gift from John Gurdon (Addgene plasmid # 30516 ; http://n2t.net/addgene:30516 ; RRID:Addgene_30516) -
For your References section:
Histone variant macroH2A confers resistance to nuclear reprogramming. Pasque V, Gillich A, Garrett N, Gurdon JB. EMBO J. 2011 May 6;30(12):2373-87. doi: 10.1038/emboj.2011.144. 10.1038/emboj.2011.144 PubMed 21552206
