Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more


(Plasmid #30525)


Item Catalog # Description Quantity Price (USD)
Plasmid 30525 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Genbank DQ836146
  • Backbone size w/o insert (bp) 3007
  • Vector type
    Mammalian Expression, Cre/Lox ; heat shock inducible, I-SceI Megabase vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    Cre recombinase tdTomato
  • Species
    synthetic, bacteriophage P1
  • Insert Size (bp)
  • GenBank ID

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Cre driven by Xenopus laevis heat shock 70 (hsp 70) promoter with CMV driven tdTomato, both in I-SceI megabase vector

Please note that there are some discrepancies between Addgene's quality control sequence and the assembled sequence from the depositor, including a H415R mutation in the downstream dimer component of tdTomato. This mutation may impact tdTomato expression.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBSHSP:Cre;CMV:tdTomato-SceI was a gift from Gerhart Ryffel (Addgene plasmid # 30525 ; ; RRID:Addgene_30525)
  • For your References section:

    Heat-shock inducible Cre strains to study organogenesis in transgenic Xenopus laevis. Roose M, Sauert K, Turan G, Solomentsew N, Werdien D, Pramanik K, Senkel S, Ryffel GU, Waldner C. Transgenic Res. 2009 Aug . 18(4):595-605. 10.1007/s11248-009-9253-4 PubMed 19266305