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HIP2
(Plasmid #31436)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 31436 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pACT2
  • Backbone manufacturer
    Clonetech
  • Backbone size w/o insert (bp) 8100
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Huntingtin Interacting protein 2
  • Alt name
    Ube2K
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    400
  • Entrez Gene
    UBE2K (a.k.a. E2-25K, HIP2, HYPG, LIG, UBC1)
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HIP2 was a gift from Rachel Klevit (Addgene plasmid # 31436 ; http://n2t.net/addgene:31436 ; RRID:Addgene_31436)
  • For your References section:

    E2-BRCA1 RING interactions dictate synthesis of mono- or specific polyubiquitin chain linkages. Christensen DE, Brzovic PS, Klevit RE. Nat Struct Mol Biol. 2007 Oct . 14(10):941-8. 10.1038/nsmb1295 PubMed 17873885