|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||32562||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2374
Modifications to backboneXhoI site has an inframe Thrombin clevage site and 6-his tag.
Vector typeSynthetic Biology
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)719
Entrez GeneeGFP (a.k.a. pPRS3a_01)
- Promoter Consitutive lac
/ Fusion Protein
- 6 His tag with Thrombin site (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site NsiI (not destroyed)
- 5′ sequencing primer pBBinF (CATCCTGAACTTATCTAGACC)
- 3′ sequencing primer pBBinR (GCAGGTCCTGAAGTTAACTAG) (Common Sequencing Primers)
Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pUCBB-ctH6-eGFP was a gift from Claudia Schmidt-dannert (Addgene plasmid # 32562 ; http://n2t.net/addgene:32562 ; RRID:Addgene_32562)
For your References section:Optimized compatible set of BioBrick vectors for metabolic pathway engineering. Vick JE, Johnson ET, Choudhary S, Bloch SE, Lopez-Gallego F, Srivastava P, Tikh IB, Wawrzyn GT, Schmidt-Dannert C. Appl Microbiol Biotechnol. 2011 Dec;92(6):1275-86. Epub 2011 Oct 28. 10.1007/s00253-011-3633-4 PubMed 22033566