Ttr:mRFP1
(Plasmid
#32607)
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Depositing Lab
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Depositing OrganizationMemorial Sloan-Kettering Cancer Center
Located in United States of America -
Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 32607 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 32607-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 32607-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepTTR1ExV3
- Total vector size (bp) 1210
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameTTR:mRFP
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SpeciesM. musculus (mouse)
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Insert Size (bp)1000
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The Ttr::RFP constructs were generated by inserting a 1-kb mRFP1 (Campbell et al., 2002)
fragment into either the NruI site of the first exon or StuI site of the second exon of
pTTR1ExV3 (Costa et al., 1990).
DNA (Catalog # 32607-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 32607-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Ttr:mRFP1 was a gift from Anna-Katerina Hadjantonakis (Addgene plasmid # 32607 ; http://n2t.net/addgene:32607 ; RRID:Addgene_32607) -
For your References section:
Transthyretin mouse transgenes direct RFP expression or Cre-mediated recombination throughout the visceral endoderm. Kwon GS, Hadjantonakis AK. Genesis. 2009 Jul;47(7):447-55. 10.1002/dvg.20522 PubMed 19415627