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pSADdeltaG-GFP-mCherry
(Plasmid #32643)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 32643 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

This item is currently unavailable outside the US without additional regulatory approval.
A non-refundable shipping export licensing fee of $85 is required to cover Addgene’s additional processing costs.

Backbone

  • Vector backbone
    pSADdeltaG
  • Backbone manufacturer
    Addgene plasmid 32634
  • Backbone size w/o insert (bp) 15428
  • Vector type
    Mammalian Expression ; Rabies virus
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    GFP
  • Insert Size (bp)
    700
  • Promoter CMV

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site NA (unknown if destroyed)
  • 3′ cloning site NA (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    mCherry
  • Insert Size (bp)
    700

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site NA (unknown if destroyed)
  • 3′ cloning site NA (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

To generate rabies virus vectors which express two genes in different segments, we
inserted the rabies transcription stop and start signals followed by mCherry ORF between
the GFP stop codon and the original transcription stop signal of GFP.
5’-TAActgcagCATGAAAAAAActAACACCCCTCCactagtcgccaccATG-3’ The underlined sequences represent the transcription stop and start signals and the stop codon of GFP and the start codon of mCherry are shown at the 5’ and 3’ ends, respectively. Then, the partial cDNA was ligated into pcDNA-SADdeltaG-GFP, resulting in pcDNASADdeltaG-GFP-mCherry.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSADdeltaG-GFP-mCherry was a gift from Edward Callaway (Addgene plasmid # 32643 ; http://n2t.net/addgene:32643 ; RRID:Addgene_32643)
  • For your References section:

    New rabies virus variants for monitoring and manipulating activity and gene expression in defined neural circuits. Osakada F, Mori T, Cetin AH, Marshel JH, Virgen B, Callaway EM. Neuron. 2011 Aug 25;71(4):617-31. 10.1016/j.neuron.2011.07.005 PubMed 21867879