Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||34559||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Backbone manufacturerPMID: 12864922
- Backbone size w/o insert (bp) 5000
Vector typeMammalian Expression, Bacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Alt namepLyn-CaM-MLCK- RhoA(Q63L)/IQp/RhoA
SpeciesH. sapiens (human)
Insert Size (bp)1200
MutationQ63L in RhoA
Entrez GeneRHOA (a.k.a. ARH12, ARHA, EDFAOB, RHO12, RHOH12)
- Promoter CMV, T7
/ Fusion Protein
- YFP (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer CMV-F, T7 (Common Sequencing Primers)
Article Citing this Plasmid
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
LYN membrane anchor - MGCIKSKGKDSATS
MLCK peptide - KRRWKKNFIAVSAANRFKKI
IQ peptide - GSAITVQRYVRGIQARAYARFL
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLyn-CaRQ was a gift from Kevin Truong (Addgene plasmid # 34559 ; http://n2t.net/addgene:34559 ; RRID:Addgene_34559)
For your References section:Ca2+-mediated synthetic biosystems offer protein design versatility, signal specificity, and pathway rewiring. Mills E, Truong K. Chem Biol. 2011 Dec 23;18(12):1611-9. 10.1016/j.chembiol.2011.09.014 PubMed 22195563