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HBM-Luc
(Plasmid #35155)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 35155 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 35155-D50 50 μg of DNA in Tris buffer $495
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DNA 35155-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pGL2-basic
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5597
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Myc promoter
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2500
  • Mutation
    Contains 2.5 kb of the c-myc minimal promoter
  • Entrez Gene
    MYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc)
  • Promoter c-myc
  • Tag / Fusion Protein
    • Luciferase (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site SacI (not destroyed)
  • 5′ sequencing primer EBV-rev
  • 3′ sequencing primer Luc_N_Rev
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Original construct in pBluescript was from E. Laufer

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid is a c-myc promoter-luciferase reporter construct designed to examine the regulation of transcription in response to conditional and constitutive Myc activation and functions as described in the associated publication.

Addgene's sequencing results identified a V13C mutation in exon 1 of c-myc when compared to GenBank reference sequence AAA59886.1

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HBM-Luc was a gift from Linda Penn (Addgene plasmid # 35155 ; http://n2t.net/addgene:35155 ; RRID:Addgene_35155)

  • For your References section:

    The Myc negative autoregulation mechanism requires Myc-Max association and involves the c-myc P2 minimal promoter. Facchini LM, Chen S, Marhin WW, Lear JN, Penn LZ. Mol Cell Biol. 1997 Jan;17(1):100-14. PubMed 8972190
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