MS2-HB
(Plasmid #35573)

• Depositing Lab: Marian Waterman
• Publication: Tsai et al Mol Cell Proteomics. 2011 Apr;10(4):M110.007385. Epub 2011 Feb 1.

Backbone

• Vector backbone: pQCXIP
• Backbone manufacturer: Qiagen
• Backbone size w/o insert (bp): 7480
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: MS2 Coat Protein
• Alt name: N55K
• Species: Bacteriophage
• Insert Size (bp): 777
• Mutation: Insert is a tail-to-head tandem placement of two MS2 coat protein open reading frames. Both MS2 contains a N55K mutation that increases protein affinity to MS2 stem loop
• Entrez Gene: cp (a.k.a. MS2g2)
• Promoter: CMV
• Tag / Fusion Protein:
  • HTBH (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Not1 (not destroyed)
• 3′ cloning site: Pac1 (not destroyed)
• 5′ sequencing primer: LNCX
• 3′ sequencing primer: IRES-R

Resource Information

• Supplemental Documents:
  • Protein sequence
• A portion of this plasmid was derived from a plasmid made by: MS2 coat protein sequence was amplified from pCT119-N55K (gift from David Peabody, University of New Mexico).
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

A small amount of plasmid dimer has been observed in Addgene's stock of this plasmid. Plasmid dimerization often does not impact plasmid function, but may reduce transformation efficiencies. You may need to screen multiple colonies to isolate the monomeric version of this plasmid. If you still have trouble isolating the monomeric version, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid.