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pUAS-Ncad-GFP
(Plasmid #35986)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 35986 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 35986-D50 50 μg of DNA in Tris buffer $495
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DNA 35986-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pUAS-EGFP
  • Backbone manufacturer
    Dev Biol. 2001 May 15;233(2):329-46
  • Modifications to backbone
    The backbone is essentially pEGFP-N1, where the CMV was deleted and the UAS cassette was inserted between HindIII-EcoR1
  • Vector type
    Gal4 inducible
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ncad
  • Species
    D. rerio (zebrafish)
  • Entrez Gene
    cdh2 (a.k.a. bib, glo, lyr, ncad, pac, wu:fb47h04)
  • Promoter 14xUAS-E1b
  • Tag / Fusion Protein
    • EGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site AgeI (not destroyed)
  • 5′ sequencing primer none
  • 3′ sequencing primer EGFP-N
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUAS-Ncad-GFP was a gift from James Jontes & Stephen Smith (Addgene plasmid # 35986 ; http://n2t.net/addgene:35986 ; RRID:Addgene_35986)

  • For your References section:

    In vivo trafficking and targeting of N-cadherin to nascent presynaptic terminals. Jontes JD, Emond MR, Smith SJ. J Neurosci. 2004 Oct 13;24(41):9027-34. 10.1523/JNEUROSCI.5399-04.2004 PubMed 15483121
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