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(Plasmid #35986)


Item Catalog # Description Quantity Price (USD)
Plasmid 35986 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Dev Biol. 2001 May 15;233(2):329-46
  • Modifications to backbone
    The backbone is essentially pEGFP-N1, where the CMV was deleted and the UAS cassette was inserted between HindIII-EcoR1
  • Vector type
    Gal4 inducible
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Species
    D. rerio (zebrafish)
  • Entrez Gene
    cdh2 (a.k.a. bi, bib, g, glo, l, laby, lyr, n-cad, nc, ncad, p, pac, parac, wu:fb47h04, znc)
  • Promoter 14xUAS-E1b
  • Tag / Fusion Protein
    • EGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site AgeI (not destroyed)
  • 5′ sequencing primer none
  • 3′ sequencing primer EGFP-N
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUAS-Ncad-GFP was a gift from James Jontes & Stephen Smith (Addgene plasmid # 35986 ; ; RRID:Addgene_35986)
  • For your References section:

    In vivo trafficking and targeting of N-cadherin to nascent presynaptic terminals. Jontes JD, Emond MR, Smith SJ. J Neurosci. 2004 Oct 13;24(41):9027-34. 10.1523/JNEUROSCI.5399-04.2004 PubMed 15483121