Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

unc5bCD4d3+4-His
(Plasmid #36151)

Loading...

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 36151 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pTT3
  • Backbone manufacturer
    Yves Durocher, NRC
  • Backbone size w/o insert (bp) 6000
  • Total vector size (bp) 7700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    unc5b
  • Alt name
    unc5b
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    1150
  • Mutation
    L2V
  • Entrez Gene
    unc5b (a.k.a. unc5h2)
  • Promoter CMV
  • Tags / Fusion Proteins
    • ratCD4d3+4 (C terminal on backbone)
    • His tag (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site AscI (not destroyed)
  • 5′ sequencing primer CMV Forward
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pTT3 was obtained from Yves Durocher, National Research Council of Canada-Biotechnology Research Institute

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid contains an intentional L2V mutation in the unc5b sequence to create a consensus Kozak sequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    unc5bCD4d3+4-His was a gift from Gavin Wright (Addgene plasmid # 36151 ; http://n2t.net/addgene:36151 ; RRID:Addgene_36151)
  • For your References section:

    A cell surface interaction network of neural leucine-rich repeat receptors. Sollner C, Wright GJ. Genome Biol. 2009;10(9):R99. Epub 2009 Sep 18. 10.1186/gb-2009-10-9-r99 PubMed 19765300