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pcDNA-mito-ZifCY1
(Plasmid #36317)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 36317 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1(+)
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5500
  • Total vector size (bp) 7400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mito-ZifCY1 genetically encoded Zn(II) sensor
  • Alt name
    4MT-Cys2His2
  • Species
    Synthetic
  • Insert Size (bp)
    1908
  • Promoter CMV
  • Tag / Fusion Protein
    • see comment

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7 Forward
  • 3′ sequencing primer BGH Reverse
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

First generation genetically encoded, ratiometric, fluorescent biosensor. Zn(II)-binding domain derived from mammalian Zif268. mito-ZifCY1 contains 4 tandem repeats of the first 29 aa of human cox subunit 8a (MSVLTPLLLRGLTGSARRLPVPRAKIHSL). HindIII restriction sites are at the 5' and 3' ends of the mitochondrial targeting sequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA-mito-ZifCY1 was a gift from Amy Palmer (Addgene plasmid # 36317 ; http://n2t.net/addgene:36317 ; RRID:Addgene_36317)
  • For your References section:

    Genetically encoded sensors to elucidate spatial distribution of cellular zinc. Dittmer PJ, Miranda JG, Gorski JA, Palmer AE. J Biol Chem. 2009 Jun 12;284(24):16289-97. Epub 2009 Apr 10. 10.1074/jbc.M900501200 PubMed 19363034