Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||36317||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5500
- Total vector size (bp) 7400
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namemito-ZifCY1 genetically encoded Zn(II) sensor
Insert Size (bp)1908
- Promoter CMV
/ Fusion Protein
- see comment
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T7 Forward
- 3′ sequencing primer BGH Reverse (Common Sequencing Primers)
Terms and Licenses
First generation genetically encoded, ratiometric, fluorescent biosensor. Zn(II)-binding domain derived from mammalian Zif268. mito-ZifCY1 contains 4 tandem repeats of the first 29 aa of human cox subunit 8a (MSVLTPLLLRGLTGSARRLPVPRAKIHSL). HindIII restriction sites are at the 5' and 3' ends of the mitochondrial targeting sequence.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA-mito-ZifCY1 was a gift from Amy Palmer (Addgene plasmid # 36317 ; http://n2t.net/addgene:36317 ; RRID:Addgene_36317)
For your References section:Genetically encoded sensors to elucidate spatial distribution of cellular zinc. Dittmer PJ, Miranda JG, Gorski JA, Palmer AE. J Biol Chem. 2009 Jun 12;284(24):16289-97. Epub 2009 Apr 10. 10.1074/jbc.M900501200 PubMed 19363034
Map uploaded by the depositor.