EYFP-Abr
(Plasmid #36414)

• Depositing Labs: John Groffen, Nora Heisterkamp
• Publication: Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. Epub 2006 Nov 20.

Backbone

• Vector backbone: pEYFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 7300
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: active BCR-related
• Alt name: ABR isoform B
• Species: H. sapiens (human)
• Insert Size (bp): 2600
• Entrez Gene: ABR (a.k.a. MDB)
• Tag / Fusion Protein:
  • EYFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (destroyed during cloning)
• 3′ cloning site: Kpn I (not destroyed)
• 5′ sequencing primer: EGFP-C
• 3′ sequencing primer: SV40pA-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The construct was made in a 3-way ligation with the ABR insert as 5' 0.4 kb BamHI-BstEII + 3' 2.2 kb BstEII-Kpn I fragment ligated into pEYFP1 digested with Bgl II x Kpn I. Non-neuronal ABR- insert starts GA TCC ATG GAG GAG GAA ....Abr. At the 3' end, the construct has the natural TAG and 110 bp of 3' UT region, terminating in what was an Xho I site. Isoform B- may contain E24G. Constructed by Jess Cunnick.