pCMMP-NGFR:LMP1-IRES-mRFP
(Plasmid #36974)

• Depositing Lab: Bill Sugden
• Publication: Pratt et al J Virol. 2012 Apr;86(8):4380-93. Epub 2012 Feb 8.

Backbone

• Vector backbone: pCMMP-MCS-IRES-mRFP
• Backbone manufacturer: Addgene plasmid #36972
• Total vector size (bp): 8596
• Vector type: Mammalian Expression, Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): Stbl3
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: NGFR:LMP1
• Species: H. sapiens (human)
• Mutation: NGFR:LMP1 chimeric receptor consists of aa 1–276 of NGF-R (extracellular and transmembrane domain) fused to aa 192–386 of LMP1 (cytoplasmic domain)
• Entrez Gene: NGFR (a.k.a. CD271, Gp80-LNGFR, TNFRSF16, p75(NTR), p75NTR)
• Entrez Gene: LMP-1 (a.k.a. HHV4_LMP-1)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NA (unknown if destroyed)
• 3′ cloning site: NA (unknown if destroyed)
• 5′ sequencing primer: pLXSN_5

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Sugden lab plasmid p3856

The NGFR-LMP1 fusion in the plasmid contains the amino terminus of NGFR fused to the carboxy terminus of LMP1. The carboxy-terminal signaling of LMP1 is conditionally activated when anti-NGFR antibody crosslinks with the NGFR
moieties. Please see associated publication (link below) for more details.