Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||37194||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4640
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth instructionsStart culture has to be freshly grow 4 to 6 hrs until O.D. around 0.6. Store it in 4oC overnight. Then it will be used to grow culture for expression.
Copy numberLow Copy
Gene/Insert nameUbiquitin G75C mutant
SpeciesH. sapiens (human)
Insert Size (bp)228
MutationGlycine 75 changed to Cystine
Entrez GeneRBX1 (a.k.a. BA554C12.1, RNF75, ROC1)
- Promoter T7
/ Fusion Protein
- No tag
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7term (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:UbG75C was a gift from Cynthia Wolberger (Addgene plasmid # 37194 ; http://n2t.net/addgene:37194 ; RRID:Addgene_37194)
For your References section:The mechanism of OTUB1-mediated inhibition of ubiquitination. Wiener R, Zhang X, Wang T, Wolberger C. Nature. 2012 Feb 22;483(7391):618-22. doi: 10.1038/nature10911. 10.1038/nature10911 PubMed 22367539