PurposeExpression of hUb in E.coli
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||61937||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4600
- Total vector size (bp) 4800
Vector typeBacterial Expression
Growth in Bacteria
Copy numberLow Copy
Gene/Insert namehuman ubiquitin
SpeciesH. sapiens (human)
Insert Size (bp)230
Entrez GeneUBC (a.k.a. HMG20)
- Promoter T7
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (unknown if destroyed)
- 3′ cloning site BamHI (unknown if destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7 term (Common Sequencing Primers)
D77 was removed via Quickchange mutagenesis.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pET3a-hUb was a gift from Chris Hill (Addgene plasmid # 61937 ; http://n2t.net/addgene:61937 ; RRID:Addgene_61937)
For your References section:Structural Basis for the Activation and Inhibition of the UCH37 Deubiquitylase. VanderLinden RT, Hemmis CW, Schmitt B, Ndoja A, Whitby FG, Robinson H, Cohen RE, Yao T, Hill CP. Mol Cell. 2015 Feb 17. pii: S1097-2765(15)00017-9. doi: 10.1016/j.molcel.2015.01.016. 10.1016/j.molcel.2015.01.016 PubMed 25702872