|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||37432||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2717
- Total vector size (bp) 16560
Vector typeGateway Entry
Growth in Bacteria
Copy numberLow Copy
SpeciesH. sapiens (human)
Insert Size (bp)13071
Entrez GeneHUWE1 (a.k.a. ARF-BP1, HECTH9, HSPC272, Ib772, LASU1, MULE, URE-B1, UREB1)
- Cloning method Gateway Cloning
- 5′ sequencing primer hHuwe1-R (GCCAAATGTTGTAGCCGAGT)
- 3′ sequencing primer hHuwe1-F (TATCAGGACTGCCCACCATT) (Common Sequencing Primers)
Terms and Licenses
Full-length cDNA constructed by sequential cloning of PCR products and adding to a partial cDNA.
The ORF sequence provided contains Q2315H and 3016del3031 amino acid mutations compared to closest reference sequence NP_113584.3).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pENTR1A-Huwe1 C4341S was a gift from Jean Cook (Addgene plasmid # 37432 ; http://n2t.net/addgene:37432 ; RRID:Addgene_37432)