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pEB1deltaC-2xEGFP shRNA resistant
(Plasmid #37925)


Item Catalog # Description Quantity Price (USD)
Plasmid 37925 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.


  • Vector backbone
  • Total vector size (bp) 4733
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
  • Species
    H. sapiens (human)
  • Mutation
    deletion after aa248, TTCCTGT=>TCCCGGT
  • Entrez Gene
    MAPRE1 (a.k.a. EB1)
  • Promoter CMV
  • Tags / Fusion Proteins
    • EGFP (C terminal on insert)
    • EGFP (C terminal on insert)

Cloning Information

Resource Information

Depositor Comments

This construct is resistant to shRNA #3.

EB1 was inserted into pEGFP-N1 via the SacI/SmaI restriction sites. The second EGFP, was then inserted via the SmaI/AgeI sites.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEB1deltaC-2xEGFP shRNA resistant was a gift from Torsten Wittmann (Addgene plasmid # 37925)
  • For your References section:

    EB1-Recruited Microtubule +TIP Complexes Coordinate Protrusion Dynamics during 3D Epithelial Remodeling. Gierke S, Wittmann T. Curr Biol. 2012 May 8;22(9):753-62. Epub 2012 Apr 5. 10.1016/j.cub.2012.02.069 PubMed 22483942