HAO1
(Plasmid #38970)

• Purpose: Bacterial expression for structure determination; may not be full ORF
• Depositing Lab: Nicola Burgess-Brown
• Publication: SGC+year (unpublished)

Backbone

• Vector backbone: pNIC28-Bsa4
• Backbone manufacturer: SGC
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Mach1
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: HAO1
• Alt name: hydroxyacid oxidase (glycolate oxidase) 1
• Alt name: PDB:2NZL
• Species: H. sapiens (human)
• Insert Size (bp): 1204
• Entrez Gene: HAO1 (a.k.a. GOX, GOX1, HAOX1)
• Tag / Fusion Protein:
  • His Tev (N terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

http://www.thesgc.org/structures/2NZL/

Addgene sequencing results found one base pair mismatch that results in W110R mutation in insert, mismatch is present in ~50% of the sample. It is unknown how this mutation impacts plasmid function, but users may be able to recover a clone without it by screening multiple colonies.