Purpose(Empty Backbone) SGC Baculovirus transfer vector with C-terminal His tag and FLAG tag, preceded by a TEV protease cleavage site. Includes sites for LIC cloning, and SacB gene for negative selection
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||39191||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
/ Fusion Protein
- TEV-His-FLAG (C terminal on backbone)
Growth in Bacteria
Growth instructionsTransform purified plasmid in DH10Bac to generate recombinant bacmid DNA
- Promoter polyhedrin
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer fBac-1 (5'-TATTCATACCGTCCCACCA-3')
- 3′ sequencing primer fBac-2 (5'-GGGAGGTTTTTTAAAGCAAGTAAA-3') (Common Sequencing Primers)
Primers for LIC cloning:
Add the following 5’ extensions to the PCR primers:
Upstream: TTAAGAAGGAGATATACTATG (ATG-initiation codon)
The purified PCR fragments are treated with T4 DNA polymerase and dGTP, then annealed to the treated vector.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pFB-CT10HF-LIC was a gift from Nicola Burgess-Brown (Addgene plasmid # 39191 ; http://n2t.net/addgene:39191 ; RRID:Addgene_39191)