pEYFP-Pds1(434)
(Plasmid
#39847)
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 39847 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepEYFP-N1
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Backbone manufacturerClontech
- Total vector size (bp) 5332
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namePds1
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Alt namePTTG1
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Alt nameSecurin
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SpeciesH. sapiens (human)
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Entrez GenePTTG1 (a.k.a. EAP1, ECRAR, HPTTG, PTTG, TUTR1)
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Tag
/ Fusion Protein
- EYFP (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer EGFP-N (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEYFP-Pds1(434) was a gift from Jonathon Pines (Addgene plasmid # 39847 ; http://n2t.net/addgene:39847 ; RRID:Addgene_39847) -
For your References section:
Human securin proteolysis is controlled by the spindle checkpoint and reveals when the APC/C switches from activation by Cdc20 to Cdh1. Hagting A, Den Elzen N, Vodermaier HC, Waizenegger IC, Peters JM, Pines J. J Cell Biol. 2002 Jun 24;157(7):1125-37. Epub 2002 Jun 17. 10.1083/jcb.200111001 PubMed 12070128
