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pAM1518
(Plasmid #40238)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 40238 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    psbAI promoter, luxCDE
  • Species
    Synechococcus elongatus PCC 7942
  • Insert Size (bp)
    1400
  • Promoter n/a

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site n/a (unknown if destroyed)
  • 3′ cloning site n/a (unknown if destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

NS1 vector

Lux Genes: luxCDE

Directs luciferase substrate synthesis in PCC7942.

psbAI promoter fragment from pAM1469 (NotI-BglII fragment) was cloned in pAM1504 cut with NotI-BamHI, upstream luxCDE. This plasmid directs aldehyde biosynthesis in cyanobacteria.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAM1518 was a gift from Susan Golden (Addgene plasmid # 40238 ; http://n2t.net/addgene:40238 ; RRID:Addgene_40238)
  • For your References section:

    Application of bioluminescence to the study of circadian rhythms in cyanobacteria. Andersson CR, Tsinoremas NF, Shelton J, Lebedeva NV, Yarrow J, Min H, Golden SS. Methods Enzymol. 2000;305:527-42. PubMed 10812624