pAM1706
(Plasmid
#40242)
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Depositing Lab
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Depositing OrganizationUniversity of California, San Diego (UCSD)
Located in United States of America -
Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 40242 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 40242-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 40242-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepAM1667
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Vector typeCyanobacteria cloning vector
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namePpsbAI
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SpeciesSynechococcus elongatus PCC 7942
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Insert Size (bp)500
- Promoter n/a
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site n/a (unknown if destroyed)
- 3′ cloning site n/a (unknown if destroyed)
- 5′ sequencing primer n/a
- 3′ sequencing primer n/a
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
NS2.1 vector
Lux Genes: luxCDE
Directs luciferase substrate synthesis in PCC7942
500bp PvuII-SalI promoter fragment of psbAI inserted into SalI-StuI digested pAM1667 (luxCDE).
DNA (Catalog # 40242-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 40242-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAM1706 was a gift from Susan Golden (Addgene plasmid # 40242 ; http://n2t.net/addgene:40242 ; RRID:Addgene_40242) -
For your References section:
Application of bioluminescence to the study of circadian rhythms in cyanobacteria. Andersson CR, Tsinoremas NF, Shelton J, Lebedeva NV, Yarrow J, Min H, Golden SS. Methods Enzymol. 2000;305:527-42. PubMed 10812624
Map uploaded by the depositor.
