MIEG-hCD4
(Plasmid #40569)

• Purpose: (Empty Backbone)
• Depositing Labs: Alexander Dent, Mark Kaplan
• Publication: Chang et al Immunity. 2005 Jun;22(6):693-703.

Backbone

• Vector backbone: pMIEG3
• Backbone manufacturer: David A. Williams (PMID: 10961859)
• Modifications to backbone: IRES EGFP of pMIEG replaced with human CD4 cDNA
• Vector type: Mammalian Expression, Retroviral
• Tag / Fusion Protein:
  • IRES hCD4 (C terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: pLXSN_5
• 3′ sequencing primer: IRES-R2 (gacggcaatatggtggaaa)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Cloned human CD4 cDNA provided by G. Alkhatib, Indiana University.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Retroviral vector MIEG-hCD4 was made by replacing the EGFP of the MIEG-EGFP vector with the hCD4 cDNA amplified from a cloned cDNA (provided by G. Alkhatib, Indiana University).