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pcDNA3.1(+)mGAT1-0-GFP
(Plasmid #41662)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 41662 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1(+)
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5428
  • Total vector size (bp) 7899
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Mus musculus GABA transporter 1
  • Alt name
    mGAT1
  • Alt name
    sodium- and chloride-dependent GABA transporter 1
  • Alt name
    solute carrier family 6 member 1
  • Species
    M. musculus (mouse), Synthetic
  • Insert Size (bp)
    2549
  • Mutation
    C-terminal hydrophobic isoleucine residue added after GFP
  • GenBank ID
    M92378.1 AAB47998.1
  • Entrez Gene
    Slc6a1 (a.k.a. A730043E01, GABATHG, GABATR, GAT-1, Gabt, Gabt1, Gat1, XT-1, Xtrp1)
  • Promoter CMV
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV-F; T7
  • 3′ sequencing primer GFPfusionRev; BGH_Rev
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

To fuse GFP to the C terminus of mGAT1, the mGAT1 open reading frame was subcloned into the HindIII and EcoRI sites of pcDNA3.1(+), and the GFP coding sequence was subcloned into the NotI and XbaI sites. A 12-residue spacer between mGAT1 and GFP was introduced by the multiple cloning site of the vector:

CGA ATT CTG CAG ATA TCC AGC ACA GTG GCG GCC GCC

R   I   L   Q   I   S   S   T   V   A   A   A

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1(+)mGAT1-0-GFP was a gift from Henry Lester (Addgene plasmid # 41662 ; http://n2t.net/addgene:41662 ; RRID:Addgene_41662)
  • For your References section:

    GABA transporter function, oligomerization state, and anchoring: correlates with subcellularly resolved FRET. Moss FJ, Imoukhuede PI, Scott K, Hu J, Jankowsky JL, Quick MW, Lester HA. J Gen Physiol. 2009 Dec;134(6):489-521. 10.1085/jgp.200910314 PubMed 19948998