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slpr genomic fragment
(Plasmid #42074)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 42074 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    slpr genomic fragment
  • Alt name
    slipper
  • Species
    D. melanogaster (fly)
  • Entrez Gene
    slpr (a.k.a. Dmel_CG2272, CG2272, D-MLK, Dmel\CG2272, JNKKK, MLK, MLK2, Mlk2, Slpr, dMLK, dMLK2)
  • Promoter Hsp70

Cloning Information

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

The slpr genomic rescue construct was generated by isolating a HindIII fragment of ∼14 kb from the P1 clone DS08402, including the MLK gene, and a neighboring annotated geneCG15339. This fragment was cloned transiently into pBluescript (Stratagene) and then moved into pCaSpeR4 vector for subsequent purification and germ-line transformation.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    slpr genomic fragment was a gift from Norbert Perrimon (Addgene plasmid # 42074 ; http://n2t.net/addgene:42074 ; RRID:Addgene_42074)
  • For your References section:

    Activation of the JNK pathway during dorsal closure in Drosophila requires the mixed lineage kinase, slipper. Stronach B, Perrimon N. Genes Dev. 2002 Feb 1;16(3):377-87. 10.1101/gad.953002 PubMed 11825878