slpr genomic fragment
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||42074||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 7862
Vector typeInsect Expression
Growth in Bacteria
Gene/Insert nameslpr genomic fragment
SpeciesD. melanogaster (fly)
Entrez Geneslpr (a.k.a. Dmel_CG2272, CG2272, D-MLK, Dmel\CG2272, JNKKK, MLK, MLK2, Mlk2, Slpr, dMLK, dMLK2)
- Promoter Hsp70
- Cloning method Unknown
- 5′ sequencing primer pCasper-F
- 3′ sequencing primer pCasper-R (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
The slpr genomic rescue construct was generated by isolating a HindIII fragment of ∼14 kb from the P1 clone DS08402, including the MLK gene, and a neighboring annotated geneCG15339. This fragment was cloned transiently into pBluescript (Stratagene) and then moved into pCaSpeR4 vector for subsequent purification and germ-line transformation.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:slpr genomic fragment was a gift from Norbert Perrimon (Addgene plasmid # 42074 ; http://n2t.net/addgene:42074 ; RRID:Addgene_42074)
For your References section:Activation of the JNK pathway during dorsal closure in Drosophila requires the mixed lineage kinase, slipper. Stronach B, Perrimon N. Genes Dev. 2002 Feb 1;16(3):377-87. 10.1101/gad.953002 PubMed 11825878