Purposea fragment containing 429 bp of the human HSPA5 promoter (-282 to +147)
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||44150||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3000
- Total vector size (bp) 5000
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert nameHSPA5 promoter
SpeciesH. sapiens (human)
Insert Size (bp)2000
Entrez GeneHSPA5 (a.k.a. BIP, GRP78, HEL-S-89n)
- Cloning method Unknown
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
a fragment containing 429 bp of the human HSPA5 promoter
(-282 to +147) relative to the transcriptional start site) was amplified from human genomic DNA by PCR and cloned into pGEM-T Easy Vector (Promega) to generate pGEM-HSPA5P
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGEM-HSPA5P was a gift from Joan Conaway (Addgene plasmid # 44150 ; http://n2t.net/addgene:44150 ; RRID:Addgene_44150)
For your References section:Endoplasmic reticulum stress-responsive transcription factor ATF6alpha directs recruitment of the Mediator of RNA polymerase II transcription and multiple histone acetyltransferase complexes. Sela D, Chen L, Martin-Brown S, Washburn MP, Florens L, Conaway JW, Conaway RC. J Biol Chem. 2012 Jun 29;287(27):23035-45. doi: 10.1074/jbc.M112.369504. Epub 2012 May 10. 10.1074/jbc.M112.369504 PubMed 22577136