pGEM-HSPA5P
(Plasmid #44150)

• Purpose: a fragment containing 429 bp of the human HSPA5 promoter (-282 to +147)
• Depositing Lab: Joan Conaway
• Publication: Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-45. doi: 10.1074/jbc.M112.369504. Epub 2012 May 10.

Backbone

• Vector backbone: pGEM
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 3000
• Total vector size (bp): 5000
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HSPA5 promoter
• Alt name: Grp78
• Species: H. sapiens (human)
• Insert Size (bp): 2000
• Entrez Gene: HSPA5 (a.k.a. BIP, GRP78, HEL-S-89n)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

a fragment containing 429 bp of the human HSPA5 promoter
(-282 to +147) relative to the transcriptional start site) was amplified from human genomic DNA by PCR and cloned into pGEM-T Easy Vector (Promega) to generate pGEM-HSPA5P