|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||44572||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Modifications to backboneN terminal GFP, tev and s peptide
Vector typeWorm Expression, Retroviral
- Promoter pie-1
/ Fusion Proteins
- GFP (N terminal on insert)
- tev (N terminal on insert)
- s peptide (N terminal on insert)
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
- Cloning method Restriction Enzyme
- 5′ sequencing primer GFP-F (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Tandem Affinity Purification plasmid - pIC26 allows a target protein to be fused at its N-terminus to the GFP-Tev-S peptide tag using the SpeI restriction site. The plasmid contains pie-1 regulatory sequences to express the tagged protein in the germline and embryo and can be integrated by ballistic bombardment of the strain DP38 using unc-119 as a transformation marker. See Cheeseman et al., Genes Dev. 2004 Sep 15;18(18):2255-68 (https://www.ncbi.nlm.nih.gov/pubmed/15371340) for more information.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pIC26 was a gift from Iain Cheeseman & Arshad Desai (Addgene plasmid # 44572 ; http://n2t.net/addgene:44572 ; RRID:Addgene_44572)
For your References section:A combined approach for the localization and tandem affinity purification of protein complexes from metazoans. Cheeseman IM, Desai A. Sci STKE. 2005 Jan 11;2005(266):pl1. 10.1126/stke.2662005pl1 PubMed 15644491