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(Plasmid #44656)


Item Catalog # Description Quantity Price (USD)
Plasmid 44656 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    GE Healtcare
  • Backbone size w/o insert (bp) 4974
  • Total vector size (bp) 7862
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    Low Copy


  • Gene/Insert name
  • Alt name
    Endoglycosidase S
  • Species
    Streptococcus pyogenes
  • Insert Size (bp)
  • Mutation
    Changed Glutamic acid 235 to Glutamine
  • GenBank ID
  • Entrez Gene
    ndoS (a.k.a. SpyM3_1568)
  • Promoter tac
  • Tag / Fusion Protein
    • Gst (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer 5' pGEX sequencing primer
  • 3′ sequencing primer 3' pGEX sequencing primer
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEXndoS(E235Q) was a gift from Mattias Collin (Addgene plasmid # 44656 ; ; RRID:Addgene_44656)
  • For your References section:

    EndoS from Streptococcus pyogenes is hydrolyzed by the cysteine proteinase SpeB and requires glutamic acid 235 and tryptophans for IgG glycan-hydrolyzing activity. Allhorn M, Olsen A, Collin M. BMC Microbiol. 2008 . 8():3. 10.1186/1471-2180-8-3 PubMed 18182097