pLenti-lxlplap
(Plasmid #45581)

• Depositing Lab: Nirao Shah
• Publication: Yang et al Cell. 2013 May 9;153(4):896-909. doi: 10.1016/j.cell.2013.04.017.

Backbone

• Vector backbone: pFUGW
• Backbone size w/o insert (bp): 9182
• Total vector size (bp): 12120
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl2
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: Green fluorescent protein
• Alt name: EGFP
• Insert Size (bp): 1170
• Promoter: Ubiquitin
• Tag / Fusion Protein:
  • Histone 2B (N terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: ACTAGTAAATTGTCCGCTAAATTCTG
• 3′ sequencing primer: CTGTGCGGGGTCAAGGGCAA

Gene/Insert 2

• Gene/Insert name: Placental alkaline phosphatase
• Alt name: PLAP
• Species: H. sapiens (human)
• Insert Size (bp): 1590
• Entrez Gene: ALPP (a.k.a. ALP, ALPI, IAP, PALP, PLAP, PLAP-1)

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: GCAGAAGAACGGCATCAAGGTGAAC
• 3′ sequencing primer: GTGGATACGCTGCTTT

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry