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(Plasmid #46644)

Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 46644 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Toshio Kitamura lab, University of Tokyo (PMID: 14585362)
  • Backbone size w/o insert (bp) 5847
  • Total vector size (bp) 7103
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
    mouse Oct3/4
  • Alt name
  • Alt name
    octamer-binding transcription factor 4
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
  • Mutation
    contains a fragment of mouse MyoD (aa1-62) fused to the N-terminus of full length mouse Oct3/4
  • GenBank ID
    NM_013633.3 NP_038661.2
  • Entrez Gene
    Pou5f1 (a.k.a. NF-A3, Oct-3, Oct-3/4, Oct-4, Oct3, Oct3/4, Oct4, Otf-3, Otf-4, Otf3, Otf3-rs7, Otf3g, Otf4)
  • Tags / Fusion Proteins
    • mouse MyoD (aa 1-62) (N terminal on insert)
    • IRES Puro (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pBMN 5' (5'-GCTTGGATACACGCCGC-3'); pMX-S1811 (5'-GACGGCATCGCAGCTTGGATACAC-3')
  • 3′ sequencing primer pCDH-rev (5'-GCATTCCTTTGGCGAGAG-3'); puro-variant-R (5'-gtgggcttgtactcggtcat-3')
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMXs-mM₃O-IP was a gift from Nobuaki Kikyo (Addgene plasmid # 46644 ; ; RRID:Addgene_46644)
  • For your References section:

    Radical acceleration of nuclear reprogramming by chromatin remodeling with the transactivation domain of MyoD. Hirai H, Tani T, Katoku-Kikyo N, Kellner S, Karian P, Firpo M, Kikyo N. Stem Cells. 2011 Sep;29(9):1349-61. doi: 10.1002/stem.684. 10.1002/stem.684 PubMed 21732495