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pMXs-hM₃O-IP
(Plasmid #46645)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 46645 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pMXs-IP
  • Backbone manufacturer
    Toshio Kitamura lab, University of Tokyo (PMID: 14585362)
  • Backbone size w/o insert (bp) 5847
  • Total vector size (bp) 7133
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hM₃O
  • Alt name
    human Oct3/4
  • Alt name
    Pou5f1
  • Alt name
    octamer-binding transcription factor 4
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1286
  • Mutation
    contains a fragment of human MyoD (aa1-62) fused to the N-terminus of full length human Oct3/4
  • GenBank ID
    NM_002701.4 NP_002692.2
  • Entrez Gene
    POU5F1 (a.k.a. OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4)
  • Tags / Fusion Proteins
    • human MyoD (aa 1-62) (N terminal on insert)
    • IRES Puro (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pBMN 5' (5'-GCTTGGATACACGCCGC-3'); pMX-S1811 (5'-GACGGCATCGCAGCTTGGATACAC-3')
  • 3′ sequencing primer pCDH-rev (5'-GCATTCCTTTGGCGAGAG-3'); puro-variant-R (5'-gtgggcttgtactcggtcat-3')
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMXs-hM₃O-IP was a gift from Nobuaki Kikyo (Addgene plasmid # 46645 ; http://n2t.net/addgene:46645 ; RRID:Addgene_46645)
  • For your References section:

    Radical acceleration of nuclear reprogramming by chromatin remodeling with the transactivation domain of MyoD. Hirai H, Tani T, Katoku-Kikyo N, Kellner S, Karian P, Firpo M, Kikyo N. Stem Cells. 2011 Sep;29(9):1349-61. doi: 10.1002/stem.684. 10.1002/stem.684 PubMed 21732495