pcDNA3 Flag Rab1a N124I
(Plasmid #46778)

• Purpose: expression of N124I Rab1a in mammalian cells
• Depositing Lab: Terry Hébert
• Publication: Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-73. Epub 2006 Sep 7.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: invitrogen
• Modifications to backbone: FLAG tag has been added.
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Rab1a
• Alt name: member RAS oncogene family
• Species: M. musculus (mouse)
• Insert Size (bp): 620
• Mutation: N124I cannot stably bind guanine nucleotide
• Entrez Gene: Rab1a (a.k.a. Gtbp, Rab-1, Rab1, Ypt1, mKIAA3012)
• Promoter: CMV
• Tag / Fusion Protein:
  • FLAG (N terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV
• 3′ sequencing primer: BGHrev

Resource Information

• Supplemental Documents:
  • Rab1a N124I sequence

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

cDNA was obtained from UMR cDNA Resource Center, www.cdna.org