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Addgene

pcDNA3 Flag Rab1a N124I
(Plasmid #46778)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 46778 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    invitrogen
  • Modifications to backbone
    FLAG tag has been added.
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Rab1a
  • Alt name
    member RAS oncogene family
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    620
  • Mutation
    N124I cannot stably bind guanine nucleotide
  • Entrez Gene
    Rab1a (a.k.a. Gtbp, Rab-1, Rab1, Ypt1, mKIAA3012)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

cDNA was obtained from UMR cDNA Resource Center, www.cdna.org

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3 Flag Rab1a N124I was a gift from Terry Hébert (Addgene plasmid # 46778 ; http://n2t.net/addgene:46778 ; RRID:Addgene_46778)
  • For your References section:

    Seven transmembrane receptor core signaling complexes are assembled prior to plasma membrane trafficking. Dupre DJ, Robitaille M, Ethier N, Villeneuve LR, Mamarbachi AM, Hebert TE. J Biol Chem. 2006 Nov 10;281(45):34561-73. Epub 2006 Sep 7. 10.1074/jbc.M605012200 PubMed 16959776