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pET12a-AL-103 FL
(Plasmid #47090)


Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 47090 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 4674
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
  • Alt name
    immunoglobulin kappa light chain variable region
  • Alt name
    amyloidosis protein
  • Species
    H. sapiens (human)
  • Entrez Gene
    IGKV3-20 (a.k.a. 13K18, A27, IGKV320)
  • Promoter T7
  • Tag / Fusion Protein
    • ompT (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET12a-AL-103 FL was a gift from Marina Ramirez-Alvarado (Addgene plasmid # 47090 ; ; RRID:Addgene_47090)
  • For your References section:

    Comparison of amyloid fibril formation by two closely related immunoglobulin light chain variable domains. Martin DJ, Ramirez-Alvarado M. Amyloid. 2010 Sep;17(3-4):129-36. doi: 10.3109/13506129.2010.530081. 10.3109/13506129.2010.530081 PubMed 21077798