pOTTC337 - pAAV EF1a DIO mCherry
PurposeAn AAV vector that expresses mCherry in a Cre-dependent manner (DIO, double floxed inverted open reading frame).
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||47636||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector backbonepAAV EF1a DIO
Backbone manufacturerKarl Deisseroth
- Backbone size w/o insert (bp) 5637
Vector typeMammalian Expression, AAV, Cre/Lox
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
- Promoter EF1a
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer EF1a_F1
- 3′ sequencing primer WPRE_R1 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
This vector has a different 5' cloning junction when compared to Karl Deisseroth's Plasmid 20299.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pOTTC337 - pAAV EF1a DIO mCherry was a gift from Brandon Harvey (Addgene plasmid # 47636 ; http://n2t.net/addgene:47636 ; RRID:Addgene_47636)
For your References section:Near-infrared fluorescent protein iRFP713 as a reporter protein for optogenetic vectors, a transgenic Cre-reporter rat, and other neuronal studies. Richie CT, Whitaker LR, Whitaker KW, Necarsulmer J, Baldwin HA, Zhang Y, Fortuno L, Hinkle JJ, Koivula P, Henderson MJ, Sun W, Wang K, Smith JC, Pickel J, Ji N, Hope BT, Harvey BK. J Neurosci Methods. 2017 Jun 1;284:1-14. doi: 10.1016/j.jneumeth.2017.03.020. Epub 2017 Apr 2. 10.1016/j.jneumeth.2017.03.020 PubMed 28380331