pCS-PesaRlux
(Plasmid #47640)

• Purpose: wt esaR promoter in pCS26
• Depositing Lab: Cynthia Collins
• Publication: Shong et al ACS Chem Biol. 2013 Apr 19;8(4):789-95. doi: 10.1021/cb3006402. Epub 2013 Feb 6.

Backbone

• Vector backbone: pCS26
• Backbone manufacturer: M. Surette (McMaster University)
• Backbone size w/o insert (bp): 9360
• Total vector size (bp): 9646
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: PesaR
• Alt name: esaR promoter
• Species: Pantoea stewartii subsp. stewartii
• Insert Size (bp): 286
• GenBank ID:
• Promoter: PesaR

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CCAGCTGGCAATTCCGA
• 3′ sequencing primer: AATCATCACTTTCGGGAA

Resource Information

• A portion of this plasmid was derived from a plasmid made by: We amplified PesaR from pTDM7 (obtained from S. von Bodman, Minogue et al., 2002, Mol. Microbiol.). We received pCS26 from M. Surette (Beeston et al., 2002, J. Bacteriol.).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

PesaR (274 bp, indicated as lowercase letters in the partial sequence labeled) is located between XhoI and BamHI sites.