Purposewt luxI promoter in pCS26
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||47641||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 9360
- Total vector size (bp) 9517
Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
Copy numberLow Copy
Alt nameluxI promoter
Insert Size (bp)157
- Promoter PluxI
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer CCAGCTGGCAATTCCGA
- 3′ sequencing primer AATCATCACTTTCGGGAA (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byWe amplified PluxI from pluxGFPuv (Collins CH et al., 2005, Mol. Microbiol.).
Terms and Licenses
PluxI (145 bp, indicated as lowercase letters in the partial sequence) was cloned into pCS-PesaRlux between XhoI and BamHI sites, replacing PesaR with PluxI. Please see pCS-PesaRlux for full plasmid backbone sequence and plasmid map.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCS-PluxIlux was a gift from Cynthia Collins (Addgene plasmid # 47641 ; http://n2t.net/addgene:47641 ; RRID:Addgene_47641)
For your References section:Directed evolution of the quorum-sensing regulator EsaR for increased signal sensitivity. Shong J, Huang YM, Bystroff C, Collins CH. ACS Chem Biol. 2013 Apr 19;8(4):789-95. doi: 10.1021/cb3006402. Epub 2013 Feb 6. 10.1021/cb3006402 PubMed 23363022