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pCS-PluxIlux
(Plasmid #47641)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 47641 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCS-PesaRlux
  • Backbone size w/o insert (bp) 9360
  • Total vector size (bp) 9517
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    PluxI
  • Alt name
    luxI promoter
  • Species
    Vibrio fischeri
  • Insert Size (bp)
    157
  • GenBank ID
  • Promoter PluxI

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CCAGCTGGCAATTCCGA
  • 3′ sequencing primer AATCATCACTTTCGGGAA
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    We amplified PluxI from pluxGFPuv (Collins CH et al., 2005, Mol. Microbiol.).
  • Terms and Licenses

Depositor Comments

PluxI (145 bp, indicated as lowercase letters in the partial sequence) was cloned into pCS-PesaRlux between XhoI and BamHI sites, replacing PesaR with PluxI. Please see pCS-PesaRlux for full plasmid backbone sequence and plasmid map.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS-PluxIlux was a gift from Cynthia Collins (Addgene plasmid # 47641 ; http://n2t.net/addgene:47641 ; RRID:Addgene_47641)
  • For your References section:

    Directed evolution of the quorum-sensing regulator EsaR for increased signal sensitivity. Shong J, Huang YM, Bystroff C, Collins CH. ACS Chem Biol. 2013 Apr 19;8(4):789-95. doi: 10.1021/cb3006402. Epub 2013 Feb 6. 10.1021/cb3006402 PubMed 23363022